Quantifying Muscle Fiber Lipid Content

For succinate dehydrogenase (SDH) staining, slides of muscle biopsies were incubated in SDH staining solution according to a previous report.21 (link) Assessment of lipid content in muscle fibers was done using the lipophilic dye boron-dipyrromethene (BODIPY) 493/503 (790389, Sigma-Aldrich). Slides were mounted with ProLong Gold Antifade Mountant with 4′,6-diamidino-2-phenylindole (DAPI) (P36931, Invitrogen). Histochemical staining was quantified by ImageJ (National Institutes of Health) software.

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Guevara-Cruz M., Godinez-Salas E.T., Sanchez-Tapia M., Torres-Villalobos G., Pichardo-Ontiveros E., Guizar-Heredia R., Arteaga-Sanchez L., Gamba G., Mojica-Espinosa R., Schcolnik-Cabrera A., Granados O., López-Barradas A., Vargas-Castillo A., Torre-Villalvazo I., Noriega L.G., Torres N, & Tovar A.R. (2020). Genistein stimulates insulin sensitivity through gut microbiota reshaping and skeletal muscle AMPK activation in obese subjects. BMJ Open Diabetes Research & Care, 8(1), e000948.

Publication 2020

BODIPY) 493/503 ProLong Gold Antifade Mountant with 4′,6-diamidino-2-phenylindole (DAPI)

Biopsies Boron Dipyrromethene Gold Lipid Muscle Succinate dehydrogenase

Corresponding Organization : Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

Other organizations : National Institute of Genomic Medicine

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Variable analysis

independent variables

SDH staining solution

dependent variables

Lipid content in muscle fibers

control variables

Incubation of muscle biopsy slides in SDH staining solution
Staining with lipophilic dye BODIPY 493/503
Mounting slides with ProLong Gold Antifade Mountant with DAPI
Histochemical staining quantification using ImageJ software

controls

No positive or negative controls were explicitly mentioned in the provided information.

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