Human prostate cancer cell lines CWR22Rv1, DU145 and LNCaP (ATCC, Manassas, VA) were cultured in RPMI 1640 (Mediatech, Herndon, VA, #15-041-CV) containing 10% fetal bovine serum (FBS; Quality Biological, Gaithersburg, MD, #100106) and penicillin/streptomycin (Mediatech, Inc., 50 IU/ml and 50 μg/ml, respectively, #30-002-CL). LNCaP cells were cultured in the presence of 0.5 nM dihydrotestosterone (DHT; Sigma, St. Louis, MO, #512-18-6). CWR22Rv1 cells were obtained in 2005 from Dr. Thomas Pretlow (Casewestern Reserve Univ.) and LNCaP and DU145 cells in 2009 from ATCC. AZD1480 (37 (link)) was provided by AstraZeneca. IL-6 producing lentivirus (LV-II-human-IL-6 virus (3.8 × 108 IU/ml) was purchased from Capital Biosciences (Rockville, MD) and recombinant IL-6 was purchased from ProSpec Protein Specialists (Brunswick, NJ, #CYT-213).
Protocol detail
Prostate Cancer Cell Line Cultivation
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Azd1480
Biological
Cell lines
Cells
Dihydrotestosterone
Human
Lentivirus
Penicillin
Prospec
Prostate cancer
Protein
Specialists
Streptomycin
Virus
Corresponding Organization : Institute of Molecular Medicine
Other organizations : AstraZeneca (United States)
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Variable analysis
independent variables
- AZD1480 (37 (link))
- IL-6 producing lentivirus (LV-II-human-IL-6 virus (3.8 × 10^8 IU/ml))
- Recombinant IL-6
- Not explicitly mentioned
- Culture media: RPMI 1640 containing 10% fetal bovine serum (FBS) and penicillin/streptomycin
- LNCaP cells cultured in the presence of 0.5 nM dihydrotestosterone (DHT)
- Not specified
- Not specified
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