Western blotting was performed as described previously (33 (link)). The primary antibodies we used were as follows: HCN1 (1:2000, ab229340, Abcam, UK), SIRT3 (1:1000, 5490, Cell Signaling Technologies, USA), SOD2 (1:1000, 13141, Cell Signaling Technology, USA), ac-SOD2 (acetyl K68) (1:1000, ab137037, Abcam, UK). We used the ECL detection system (Beyotime Institute of Biotechnology, China) and ImageJ software for protein quantification.
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