The PET imaging studies in this work were conducted according to literatures.30 (link), 52 (link) T1-weighted MR anatomical images were acquired on a 3.0 T scanner (GE Discovery 750, Milwaukee, USA). The rhesus was anesthetized with ketamine (10 mg/kg; intramuscular injection) and placed into the scanner in the supine position. A circular 32-channel array head coil was placed on top of the monkey’s head. The whole-brain images were acquired with a 3D Bravo T1 sequence.
A male rhesus (weight range 5.94–6.16 kg) underwent PET scan (GE Discovery Elite 690, USA). The rhesus was anesthetized with ketamine (10 mg/kg; i.m.), placed into the scanner, and maintained in light narcosis with 2% isoflurane and 98% oxygen. The body of the subject was in the supine position. A solution of [18F]10 or [18F]15 (3.34–3.95 mCi) in saline was injected into the monkey via a flexible percutaneous venous catheter. The baseline PET/CT scan were repeated twice within 10 days. For the blocking study, the blocking reagent JNJ-42226314 (0.9 mg/kg) was injected intravenously, followed by the injection of [18F]15 .
A male rhesus (weight range 5.94–6.16 kg) underwent PET scan (GE Discovery Elite 690, USA). The rhesus was anesthetized with ketamine (10 mg/kg; i.m.), placed into the scanner, and maintained in light narcosis with 2% isoflurane and 98% oxygen. The body of the subject was in the supine position. A solution of [18F]
