Myoblast differentiation was examined according to previously published protocols [18 (link)], and samples were imaged on INCell Analyzer 6000 (GE Healthcare), equipped with 10×/0.45 NA Plan Apo objective and 2048 × 2048 sCMOS camera. Twenty-five fields with five z-planes were acquired. Individual focal plane image for each z-series was automatically selected based on sharpness. Image analysis algorithm and PCR is described in Supplementary Methods .
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Myoblast Differentiation Imaging Protocol
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Myoblast
Corresponding Organization : Ontario Institute for Cancer Research
Other organizations : Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, National Cancer Institute, Center for Cancer Research, Hospital for Sick Children, National Center for Advancing Translational Sciences, National Institutes of Health
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- Myoblast differentiation
- Myoblast differentiation
- Previously published protocols [18 (link)]
- INCell Analyzer 6000 (GE Healthcare), equipped with 10×/0.45 NA Plan Apo objective and 2048 × 2048 sCMOS camera
- Twenty-five fields with five
z -planes were acquired - Individual focal plane image for each
z -series was automatically selected based on sharpness - Image analysis algorithm and PCR is described in
Supplementary Methods
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