Orthotopic Injection of DIPG Neurospheres

This procedure was carried out as described (9 (link)). Briefly, we prepared a single-cell suspension of luciferase-transduced SU-DIPG-XIII-luc neurospheres (pLenti PGK V5-LUC Neo (w623-2); Addgene, plasmid #21471), and injected 10⁵ cells (50,000 cells/μL) into the fourth ventricle/pons of immunocompromised NOD-SCID-gamma (NSG) (strain 005557; The Jackson Laboratory), cold-anesthetized, P3 mouse pups through a 30-gauge burr hole (stereotactic coordinates: 3 mm posterior to the lambda suture and 3 mm deep).

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Zhang Q., Yang L., Liu Y.H., Wilkinson J.E, & Krainer A.R. (2023). Antisense oligonucleotide therapy for H3.3K27M diffuse midline glioma. Science translational medicine, 15(691), eadd8280.

Publication 2023

pLenti PGK V5-LUC Neo NOD-SCID-gamma (NSG)

Burr hole Cells Cold Dipg Fourth ventricle Gamma Luciferase Mouse Plasmid Pons Scid Strain Suture

Corresponding Organization : Cold Spring Harbor Laboratory

Other organizations : Stony Brook University, University of Michigan–Ann Arbor

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Variable analysis

independent variables

Injection of 10^5 luciferase-transduced SU-DIPG-XIII-luc neurospheres cells (50,000 cells/μL) into the fourth ventricle/pons of immunocompromised NOD-SCID-gamma (NSG) mouse pups

dependent variables

Not explicitly mentioned

control variables

Immunocompromised NOD-SCID-gamma (NSG) mouse pups
Stereotactic coordinates: 3 mm posterior to the lambda suture and 3 mm deep

positive controls

Not specified

negative controls

Not specified

Annotations

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