Investigating Cell Migration Signaling

Antibodies include mouse monoclonal for HA-probe (F-7) (sc-7392), β-actin (C4) (sc-47778), and GFP (sc-101525) and rabbit polyclonal for FAK (sc-557) (Santa Cruz Biotechnology Inc, Dallas, TX, USA), CXCR4 rabbit polyclonal Ab (Ab-2074) (Abcam, Cambridge, MA, USA), and pY397-FAK rabbit monoclonal Ab (Invitrogen, 44625G, Carlsbad, CA, USA), and vimentin mouse monoclonal Ab (550513) (BD Pharmingen, San Jose, CA, USA). Anti-KLF8 rabbit polyclonal Ab was described previously [18 (link), 19 (link)]. Peroxidase substrate kit (DAB) (SK-4100) was purchased from Vector laboratories Inc. (Burlingame, CA, USA). The CXCR4 inhibitor AMD3100 (or octahydrochloride hydrate) (A5602) and the FAK inhibitor PF573228 (PZ0117) were purchased from Sigma (St. Louis, MO, USA). Recombinant human CXCL12 (300–28A) was purchased from Peprotech (Rocky Hill, CT, USA).