Antibodies to proliferating cell nuclear antigen, ribosomal P, recombinant Ro 52/TRIM21, native SSA/Ro 60, SSB/La, Sm, and U1 RNP were detected using the extractable nuclear antigen FIDIS Connective Profile, kit 13 addressable laser bead immunoassay (TheraDiag) on a Luminex 200 flow luminometer, according to the manufacturer’s instructions, and using MLX-Booster software. Other autoantibodies, such as IgG anticardiolipin, IgG anti–β2-glycoprotein 1, and lupus anticoagulant, were measured in a central laboratory as previously described (28 (link)). ANA IIF patterns were classified according to the new ICAP standards (
Autoantibody Detection in Rheumatic Diseases
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Corresponding Organization :
Other organizations : University of Calgary, McGill University Health Centre, Dalhousie University, Queen Elizabeth II Health Sciences Centre, University of Toronto, Toronto Western Hospital, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, University of Birmingham, Hanyang University Seoul Hospital, Cedars-Sinai Medical Center, University of California, Los Angeles, Oklahoma Medical Research Foundation, University College London, SUNY Downstate Health Sciences University, State University of New York, Johns Hopkins Medicine, Johns Hopkins University, NIHR Manchester Biomedical Research Centre, University of Manchester, Manchester Academic Health Science Centre, University of North Carolina at Chapel Hill, Université Laval, Mount Sinai Hospital, University Health Network, Reykjavík University, National University Hospital of Iceland, Northwestern University, St Thomas' Hospital, King's College London, Feinstein Institute for Medical Research, University of Alabama at Birmingham, Allegheny Health Network, Lund University, Hairmyres Hospital, University of Amsterdam, Hospital Clínic de Barcelona, Hospital de Cruces, University of the Basque Country, Emory University, University of California, San Diego, Istanbul University, Medical University of South Carolina, University of Manitoba, Copenhagen University Hospital, Rigshospitalet, New York University Langone Orthopedic Hospital, Spitäler Schaffhausen, New York University
Variable analysis
- None explicitly mentioned
- Presence of anti-dsDNA antibodies
- Antibodies to proliferating cell nuclear antigen, ribosomal P, recombinant Ro 52/TRIM21, native SSA/Ro 60, SSB/La, Sm, and U1 RNP
- IgG anticardiolipin, IgG anti–β2-glycoprotein 1, and lupus anticoagulant
- ANA IIF patterns
- None explicitly mentioned
- None specified
- None specified
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