Human SkBr3 (HTB‐30) and BT474 (HTB‐20) breast cancer cell lines were obtained from the American Type Culture Collection (ATCC). Lapatinib‐resistant (LR) clones were generated from long‐term treatment with Lapatinib (GlaxoSmithKline).24 (link), 25 (link) All cell lines were cultured in Dulbecco's modified Eagle medium/F12 (SH30004.04, HyClone) supplemented with 10% fetal bovine serum (10437‐028, Gibco), 1% penicillin‐streptomycin (SH40003.01, HyClone) at 37°C in an humidified atmosphere of 95% air and 5% CO2. LR clones were maintained in the presence of 1 μmol/L Lapatinib.
Protocol detail
Generating Lapatinib-Resistant Breast Cancer Cell Lines
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Atmosphere
Breast cancer cell lines
Cell lines
Clones
Cultured medium
Eagle
Fetal bovine serum
Human
Lapatinib
Long term treatment
Penicillin
Streptomycin
Corresponding Organization : Asia University
Other organizations : Tunghai University
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Variable analysis
independent variables
- Long-term treatment with lapatinib (GlaxoSmithKline)
- Generation of lapatinib-resistant (LR) clones
- Cell lines: SkBr3 (HTB-30) and BT474 (HTB-20) breast cancer cell lines
- Cell culture conditions: Dulbecco's modified Eagle medium/F12 supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin, maintained at 37°C in a humidified atmosphere of 95% air and 5% CO2
- Maintenance of LR clones: Presence of 1 μmol/L lapatinib
- Positive control: Not specified
- Negative control: Not specified
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