Isolation of Naive CD4+ T Cells

CD4⁺ T cells were isolated by using anti-mouse CD4-magnetic beads (BD Biosciences) [24 (link)]. CD4⁺CD62L⁺ naïve T cells were isolated by using the CD4⁺CD62L⁺ T cell isolation kit from Miltenyi Biotec according to the instructions provided by the manufacturer [25 (link)].

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Wu W., Liu H.P., Chen F., Liu H., Cao A.T., Yao S., Sun M., Evans-Marin H.L., Zhao Y., Zhao Q., Duck L.W., Elson C.O., Liu Z, & Cong Y. (2016). Commensal A4 bacteria inhibit intestinal Th2-cell responses through induction of dendritic cell TGF-β production. European journal of immunology, 46(5), 1162-1167.

Publication 2016

anti-mouse CD4-magnetic beads CD4+CD62L+ T cell isolation kit

Cd4 t cell Cd62l Isolation Mouse

Corresponding Organization : The University of Texas Medical Branch at Galveston

Other organizations : Shanghai Tenth People's Hospital, Tongji University, Qilu Hospital of Shandong University, University of Alabama at Birmingham

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Variable analysis

independent variables

Isolation of CD4+ T cells using anti-mouse CD4-magnetic beads
Isolation of CD4+CD62L+ naïve T cells using the CD4+CD62L+ T cell isolation kit from Miltenyi Biotec

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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