Western Blot Protein Analysis

Western blot analysis was performed as described earlier^{28 (link),29 (link)}. The primary antibodies used were anti-AKT, anti-S473 phopsho-AKT, anti-GAPDH, anti-PARP, anti-Caspase-3, anti-ERK1/2, anti-phospho 44/42 ERK1/2, anti-phospho-SAPK/JNK, anti-SAPK/JNK, anti-SP1, anti-LC3B (Cell Signalling Technology), anti-p24 (Cat No. 6457, NIH), anti-IKBα (Santa Cruz Biotechnology) The secondary antibodies used were anti-rabbit/mouse-HRP conjugated (Jackson Immuno Research).

Free full text: Click here

Raja R., Lata S., Trivedi S, & Banerjea A.C. (2018). Serum deprivation/starvation leads to reactivation of HIV-1 in latently infected monocytes via activating ERK/JNK pathway. Scientific Reports, 8, 14496.

Publication 2018

anti-GAPDH anti-PARP anti-Caspase-3 anti-ERK1/2 anti-phospho-SAPK/JNK anti-SAPK/JNK anti-SP1 anti-LC3B anti-IKBα anti-rabbit/mouse-HRP conjugated

Antibodies Caspase 3 Erk1 Gapdh Mouse Rabbit Western blot analysis

Corresponding Organization :

Other organizations : National Institute of Immunology

Top 5 similar protocols

Protocol cited in 2 other protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of AKT, phospho-AKT, GAPDH, PARP, Caspase-3, ERK1/2, phospho-ERK1/2, phospho-SAPK/JNK, SAPK/JNK, SP1, LC3B, p24, IKBα

control variables

None explicitly mentioned

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!