Scratch test for registering the capacity of PRGF and PRF compounds to induce wound healing was used the test for monitoring the actual fibroblasts migration on a standard gap (14 (link)). Fibroblasts were seeded at a density of 0.33×106 cells/IBIDI plate and 24 h of cultivation at 37°C in 5% CO2, the silicon frame was removed displaying a standard gap of 500 µm (Fig. 3). The plates were washed thoroughly with PBS to remove any debris. Fibroblasts in the presence of 3 ml complete medium with/without 10% serum (controls) or in the presence of the tested compounds were incubated and migration registered by video capture in BioStation (Nikon Corporation, Tokyo, Japan).