Immunohistochemistry was performed using mouse monoclonal anti-Ki67 and anti-CD31 (Dako, Agilent Technologies, Glostrup, Denmark; available on: http://www.dako.com) [45 (link),46 (link),47 (link),48 (link)]. The percentage of Ki67+ cells in the basal layer of the epidermis and in the outer root sheath of hair follicles, and the number of vessels per mm2 were calculated according to morphometric criteria.
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