The blood taken from the heart and veins of the deeply anesthetized rats was transferred to yellow-capped biochemistry tubes without anticoagulant. These blood samples were centrifuged at 4000 revolutions per minute for 10 min, and the plasma was transferred to Eppendorf tubes to be stored at −80°C until the day of the experiment. On the experiment day, these samples were thawed at room temperature, and the parameters were analyzed.
Our study protocol was carried out in 3 steps. In the first step, basal biochemical parameters were measured using Siemens brand commercial kits. Alanine aminotransferase (ALT), urea, and creatinine (Cr) parameters were measured in Atellica Solution device; pro-brain natriuretic peptide (pro-BNP) was measured in the AQT90 Flex device, and troponin I and creatine kinase (CK-MB) values were measured in the Advia Centaur XP immunoassay System device. In the second step, caspase 3/7 and 8-hydroxy-deoxyguanosine (8-OHdG) (BT LAB, Cat. no E0031Ra) were measured with enzyme-linked immunosorbent assay. Total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) values were measured. Total antioxidant status and TOS levels of the samples were measured using Rel Assay brand commercial kits. Total antioxidant status levels in tissues were expressed as Trolox equivalent/L, and TOS results as μmol H2O2 equivalent/L.19 (link),20 (link) Oxidative stress index values, an indicator of oxidative stress, were expressed in arbitrary units (AU). In the third stage, the cardiac tissues of the rats were examined histopathologically. Finally, all data were analyzed statistically.
Our study protocol was carried out in 3 steps. In the first step, basal biochemical parameters were measured using Siemens brand commercial kits. Alanine aminotransferase (ALT), urea, and creatinine (Cr) parameters were measured in Atellica Solution device; pro-brain natriuretic peptide (pro-BNP) was measured in the AQT90 Flex device, and troponin I and creatine kinase (CK-MB) values were measured in the Advia Centaur XP immunoassay System device. In the second step, caspase 3/7 and 8-hydroxy-deoxyguanosine (8-OHdG) (BT LAB, Cat. no E0031Ra) were measured with enzyme-linked immunosorbent assay. Total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) values were measured. Total antioxidant status and TOS levels of the samples were measured using Rel Assay brand commercial kits. Total antioxidant status levels in tissues were expressed as Trolox equivalent/L, and TOS results as μmol H2O2 equivalent/L.19 (link),20 (link) Oxidative stress index values, an indicator of oxidative stress, were expressed in arbitrary units (AU). In the third stage, the cardiac tissues of the rats were examined histopathologically. Finally, all data were analyzed statistically.
