Template-Switch Reaction with PEG

The template-switch reaction was conducted following the procedure previously described [43] .
Additionally, to enhance the efficiency of this reaction, we incorporated polyethylene glycol (PEG 8000; Promega) at a concentration of 10% w/v in each reaction, as previously mentioned. This inclusion of polyethylene glycol aids in promoting optimal reaction conditions acting as a molecular crowding agent and thereby facilitates the successful transformation of ssDNA into dsDNA.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Emili E., Rodríguez-Fernández D., Pérez-Posada A., García-Castro H., & Solana J. (2024). Multiplex single-cell analysis of serotonergic neuron function in planarians reveals widespread effects in diverse cell types.

Publication 2024

Corresponding Organization : Oxford Brookes University

Top 5 similar protocols

Variable analysis

independent variables

Incorporation of polyethylene glycol (PEG 8000; Promega) at a concentration of 10% w/v in each reaction

dependent variables

Efficiency of the template-switch reaction

control variables

Procedure for the template-switch reaction as previously described [43]

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!