ChIP Assay and Quantitative PCR Protocol

Antibodies were described before [27 (link)] or are as follows: anti-HA [12CA5] (Roche, 11583816001, for western); anti-HA (Abcam, ab9110, for ChIP); anti-Myc (Santa Cruz Biotechnology, sc-764x) anti-Oct4 (Santa Cruz Biotechnology, sc-8628x). RNA extraction and ChIP assays were done as described [27 (link)]. qPCR was performed with SYBR Advantage qPCR Premix (Clontech) on a ViiA7 Real-Time PCR System (Applied Biosystems). Primers used are listed in Table 1.

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Yang Z., Augustin J., Hu J, & Jiang H. (2015). Physical Interactions and Functional Coordination between the Core Subunits of Set1/Mll Complexes and the Reprogramming Factors. PLoS ONE, 10(12), e0145336.

Publication 2015

ab9110 anti-Myc anti-Oct4 SYBR Advantage qPCR Premix ViiA7 Real-Time PCR System

Antibodies Chip Chip assays Oct4 Primers

Corresponding Organization : University of Alabama at Birmingham

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Variable analysis

independent variables

Anti-HA [12CA5] (Roche, 11583816001, for western)
Anti-HA (Abcam, ab9110, for ChIP)
Anti-Myc (Santa Cruz Biotechnology, sc-764x)
Anti-Oct4 (Santa Cruz Biotechnology, sc-8628x)

dependent variables

RNA extraction and ChIP assays

control variables

SYBR Advantage qPCR Premix (Clontech)
ViiA7 Real-Time PCR System (Applied Biosystems)
Primers used are listed in Table 1

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