Shrimp Viral Isolation and Metagenomics

Samples of shrimp Litopenaeus vannamei (No. 20141215) were collected from a farmed pond with high mortality in a farm in Taizhou of Zhejiang Province in December 2014 for viral isolation and metagenomic analysis. For nested PCR tests, L. vannamei, Fenneropenaeus chinensis, Macrobrachium rosenbergii, and Marsupenaeus japonicas were collected from shrimp farms in the coastal provinces of China during 2014 and 2015. Additionally, healthy juveniles of L. vannamei (mean body weight, 3 g) for challenge test were purchased from a shrimp farm in Weifang of Shandong Province, China. The shrimp samples were demonstrated to be free of WSSV, IHHNV, VPAHPND, YHV and TSV by PCR or RT-PCR methods recommended by the World Organization for Animal Health^{11 ,12} .