Immunoblotting Antibodies for Yeast Proteome

α-Hsf1 (1:5000; Supplementary Fig. 4a and Supplementary Note 3), α-Sch9 (1:500; Supplementary Fig. 6a), α-Sse1 (1:10,000)^{9 (link)}, α-Ssb (1:5000)^{58 (link)}, α-Rpl35 (1:10,000)^{4 (link)}, α-Rpl17 (1:10,000)^{58 (link)}, α-Rpl19 (1:10,000)^{4 (link)}, α-Rps6 (1:10,000; Supplementary Fig. 6b), α-Rps9 (1:10,000)^{58 (link)}, α-Ssa (1:10,000)^{9 (link)}, α-Pgk1 (1:5000; Supplementary Fig. 6c), and α-Rps20 (1:10,000; Supplementary Fig. 6d) were rabbit polyclonal antibodies (Eurogentec, Rospert lab antibody collection). The rabbit polyclonal α-Maf1 (1:5000) was a kind gift from Olivier Lefebvre (Commissariat à l’Energie Atomique, France). α-phospho-Sch9-T570 (1:5000)^{24 (link)} was kindly provided by Robbie Loewith (University of Geneva). Commercially purchased antibodies were α-FLAG (1:10,000; Agilent 200471), α-GFP (1:5000; Life Technologies A11122), α-rabbit HRP (1:10,000; Pierce 31460) and α-mouse HRP (1:10,000; Santa Cruz SC-2005). Immunoblots were detected by enhanced chemiluminescence^{59 (link)}. Uncropped scans of the most important immunoblots are shown in Supplementary Figs 7–12.