ZDF rat kidney and urine samples were provided by Epigen Inc. to verify that kidney spatial adenine correlated with the targeted urine adenine assay. C57BL/6J, db/m, and db/db mice were obtained from The Jackson Laboratory. C57BL/6J mice were administered adenine for 4 weeks in drinking water before sacrifice, and tissues and blood samples were harvested at University of Texas Health Science Center at San Antonio. db/m and db/db mice were administered vehicle or MTDIA MTAP inhibitor for a period of 8 weeks from week 10 to week 18. An Albumin ELISA kit (catalog E101 and E90-134, Bethyl Laboratories Inc.) and creatinine colorimetric kit (catalog ADI-907-030A, Enzo Life Sciences Inc.) were used for the urinary ACR. Serum cystatin C was measured by the Quantikine ELISA kit (catalog MSCTC0, R&D Systems). Plasma creatinine and metabolites in kidney tissue were measured by Zip-Chip-mass spectrometry as previously described (12 (link)). Urine and kidney KIM-1 was measured by ELISA (catalog DY1817, R&D System).
Other organizations :
The University of Texas Health Science Center at San Antonio, The University of Texas at San Antonio, Icahn School of Medicine at Mount Sinai, University of Colorado Anschutz Medical Campus, University of Michigan–Ann Arbor, Khoo Teck Puat Hospital, National Institute of Diabetes and Digestive and Kidney Diseases, University Health Network, University of California, San Diego, Research Institute of Molecular Pathology, University of California, Los Angeles, Boston University, Seattle University, University of Washington, ETH Zurich, University of Pennsylvania, Epigen Biosciences (United States), Cleveland Clinic Lerner College of Medicine, University Medical Center Groningen, University of Groningen, Pacific Northwest National Laboratory, Environmental Molecular Sciences Laboratory, European Molecular Biology Laboratory
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