GC-MS Analysis of Methanolic Extract

The GC-MS analysis was performed on the methanolic extract MUSC 125 following the method of Tan, et al. [46 (link)] which utilized an Agilent Technologies 6980N fitted with 5979 Mass Selective Detector and a HP-5 MS (5% phenyl methyl siloxane) capillary column to carry helium gas at the rate of 1 mLs⁻¹. Heat was gradually applied until 40 °C was reached whilst keeping it constant for 10 min; then, it was increased by 3 °C every minute until the peak temperature of 250 °C was reached keeping, it constant for 5 another minutes. The mass spectrometry was functioning at 70 eV. Individual compounds detected by GC-MS were matched with the NIST 05 reference library.