Glucose-Induced miR-320-3p Regulation in Diabetes

The miR-320-3p has been associated with the regulation of Glucose-Induced Gene Expression in Diabetes (Feng and Chakrabarti, 2012 (link)). At sacrifice, rat pup was sampled of one blood drop to determine the total blood glucose by AccuChekH Active (Roche-Diagnostics GmbH, Mannheim, Germany), and blood plasma was collected on EDTA tubes (Wang et al., 2012 (link)), after centrifugation at 2,000 × g for 10 min and stored at −80°C. Cholesterol (Cholesterol FS^*, DiaSys Diagnostic Systems GmbH), Triglycerides (Triglycerides FS^*, DiaSys Diagnostic Systems GmbH), and Non-Essential Free fatty acids (NEFA FS^*, DiaSys Diagnostic Systems GmbH, Holzheim, Germany) were measured on rat plasma.

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Beuzelin D., Pitard B, & Kaeffer B. (2019). Oral Delivery of miRNA With Lipidic Aminoglycoside Derivatives in the Breastfed Rat. Frontiers in Physiology, 10, 1037.

Publication 2019

Cholesterol FS*, Triglycerides FS*, NEFA FS*,

Blood Blood glucose Centrifugation Cholesterol Diabetes Diagnostic Edta Essential fatty acids Glucose Nefa Plasma Regulation of gene expression Triglycerides

Corresponding Organization :

Other organizations : Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers, Inserm, Université d'Angers, Nantes Université

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Variable analysis

independent variables

Mimic the regulation of Glucose-Induced Gene Expression in Diabetes caused by miR-320-3p

dependent variables

Total blood glucose
Cholesterol
Triglycerides
Non-Essential Free fatty acids

control variables

Rat pup
Blood sampling and plasma collection
Centrifugation at 2,000 × g for 10 min
Storage at -80°C

controls

Positive control: Not specified
Negative control: Not specified

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