Lipid Profiling by Negative Ion LC/MS

Negative ion electrospray ionization LC/MS analysis of lipid extracts was performed on a Thermo Scientific (San Jose, CA, USA) Vantage TSQ mass spectrometer with Thermo Accela UPLC operated by Xcalibur software. Lipids were separated on a Restek 150 × 2.1 mm (5 µm particle size) Viva C₄ column under established conditions. The tetramyristoyl CL internal standard (m/z 1,240, [M-H]^-) elutes at 13.6 minutes while tetralinoleoyl CL (m/z 1,448, [M-H]^-) elutes at 14.4 minutes. Complete details of CL analysis by LC/MS are provided elsewhere^{[16 (link), 21 (link)–24 (link)]}_.

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Ikon N., Hsu F.F., Shearer J., Forte T.M, & Ryan R.O. (2017). Evaluation of cardiolipin nanodisks as lipid replacement therapy for Barth syndrome. Journal of Biomedical Research, 32(2), 107-112.

Publication 2017

Vantage TSQ Accela UPLC Viva C4 column

Lipids

Corresponding Organization : University of Nevada, Reno

Other organizations : Washington University in St. Louis

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Variable analysis

independent variables

Lipid extracts

dependent variables

Lipid separation and elution times

control variables

Tetramyristoyl CL internal standard (m/z 1,240, [M-H]-) eluting at 13.6 minutes
Tetralinoleoyl CL (m/z 1,448, [M-H]-) eluting at 14.4 minutes

positive controls

Tetramyristoyl CL internal standard (m/z 1,240, [M-H]-)
Tetralinoleoyl CL (m/z 1,448, [M-H]-)

negative controls

Not explicitly mentioned

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