Protocol detail

Glycine-mediated cLTP Induction and Wnt Blockade

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LTP was induced in cultured hippocampal neurons with a glycine-mediated form of cLTP as previously described (Fortin et al., 2010 (link), Stamatakou et al., 2013 (link)). To block Wnts, a combination of recombinant Sfrp1 (2.5 μg/mL; R&D Systems, Abingdon, UK) and Sfrp3 (250 ng/mL; R&D Systems) was used throughout the induction of cLTP and during the incubation with control solution. See Supplemental Experimental Procedures for more information.

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McLeod F., Bossio A., Marzo A., Ciani L., Sibilla S., Hannan S., Wilson G.A., Palomer E., Smart T.G., Gibb A, & Salinas P.C. (2018). Wnt Signaling Mediates LTP-Dependent Spine Plasticity and AMPAR Localization through Frizzled-7 Receptors. Cell Reports, 23(4), 1060-1071.

Publication 2018

Sfrp1 Sfrp3

Block Glycine Neurons Sfrp1

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Protocol cited in 4 other protocols

Variable analysis

independent variables

Induction of LTP in cultured hippocampal neurons with a glycine-mediated form of cLTP
Blocking of Wnts with a combination of recombinant Sfrp1 (2.5 μg/mL) and Sfrp3 (250 ng/mL)

dependent variables

Measured outcomes (not explicitly specified)

control variables

Incubation with control solution

controls

Positive control: Induction of LTP in cultured hippocampal neurons with a glycine-mediated form of cLTP, as previously described
Negative control: Blocking of Wnts with a combination of recombinant Sfrp1 (2.5 μg/mL) and Sfrp3 (250 ng/mL) during the induction of cLTP and incubation with control solution

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