Enzymatic Synthesis of Puerarin Ester

Puerarin ester was synthesized via enzymatic synthesis using lipase (Figure 10). The enzymatic synthesis of puerarin ester was performed using a shaking incubator while maintaining a working volume of 10 mL in a 50 mL serum bottle. Puerarin (10 mM) and the acyl donor (10–250 mM) were dissolved in an organic solvent and placed in a serum bottle. A certain concentration of enzyme (1–25 g/L) was then added. A serum bottle containing the enzyme-containing reaction solution was sealed to prevent evaporation. The reaction was then performed at the same temperature at 180 rpm for 48 h in a shaking incubator. Following the reaction, 100 μL of the reaction solution was collected, diluted 10 times with methanol, filtered, and analyzed by HPLC. All experiments were repeated at least in triplicate, and the data are presented as the mean ± standard deviation of the measured or calculated values.