Chronic Corticosterone Induces Depression-like Behavior

Corticosterone (CORT) (27840, Sigma-Aldrich NV) was dissolved in b-cyclodextrin solution (4% in tap water, C4767, Sigma-Aldrich NV) to a concentration of 36.5 mg/L [51 (link)] and provided in drinking water over 4 weeks. This dose has been shown to induce depression and anxiety-like features in different mouse strains including blunting of corticosterone response following an acute stressor [51 (link), 52 (link)], while increasing the corticosterone levels less than what is observed after acute or chronic stress exposure [53 (link)–55 (link)]. Treatment was maintained during behavioural testing to prevent rebound effects. At the end of the behavioural tests, dorsal hippocampus was isolated and snap frozen.

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Van Looveren K., Van Boxelaere M., Callaerts-Vegh Z, & Libert C. (2019). Cognitive dysfunction in mice lacking proper glucocorticoid receptor dimerization. PLoS ONE, 14(12), e0226753.

Publication 2019

C4767

Anxiety Behavioural tests Corticosterone Cyclodextrin Frozen Hippocampus Mouse Strains

Corresponding Organization : VIB-KU Leuven Center for Brain & Disease Research

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Variable analysis

independent variables

Corticosterone (CORT) dissolved in β-cyclodextrin solution (4% in tap water) to a concentration of 36.5 mg/L and provided in drinking water over 4 weeks

dependent variables

Depression and anxiety-like features in different mouse strains
Corticosterone response following an acute stressor
Corticosterone levels

control variables

Treatment was maintained during behavioural testing to prevent rebound effects

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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