CaMKII isoforms and phosphoCaMKII (pCaMKII) were detected by indirect immunofluorescence as described in12 (link). For a list of retrieval methods, antibodies and dilutions please refer to Supplementary Table 2. All images were acquired using identical settings on an Olympus BX61 microscope and consistently modified for best rendering using Adobe Photoshop. The fluorescent and dapi images of the same field were superimposed, or the fluorescent and Nomarski images of the same field were inverted and superimposed (subtraction).
Nalesso G., Thorup A.S., Eldridge S.E., De Palma A., Kaur A., Peddireddi K., Blighe K., Rana S., Stott B., Vincent T.L., Thomas B.L., Bertrand J., Sherwood J., Fioravanti A., Pitzalis C, & Dell’Accio F. (2021). Calcium calmodulin kinase II activity is required for cartilage homeostasis in osteoarthritis. Scientific Reports, 11, 5682.
Other organizations :
University of Surrey, William Harvey Research Institute, Queen Mary University of London, MRC Clinical Trials Unit at UCL, University College London, Imperial College London, University of Oxford, Otto-von-Guericke University Magdeburg, University Hospital Münster, Azienda Ospedaliera Universitaria Senese
Detection of CaMKII isoforms and phosphoCaMKII (pCaMKII) by indirect immunofluorescence
control variables
Acquisition of all images using identical settings on an Olympus BX61 microscope
Consistent modification of images for best rendering using Adobe Photoshop
controls
Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned
Annotations
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