Bioreactor batch cultures were performed in a Sartorius Biostat B plus bioreactor in 1 liter of M9 medium with 90 mMeqC of glucose and/or xylose mixes. The temperature was set at 37°C and pH at 7. Nonlimiting aeration conditions were obtained with an airflow set at 0.35 liter · min−1 and adaptation of stirring to maintain pO2 of >20%. Growth was assessed by OD600 measurement at 30-min intervals with a LibraS4 spectrophotometer (Biochrom, UK). Glucose, xylose, and acetate concentrations were quantified by high-performance liquid chromatography (HPLC) (Agilent, USA) with an HPX 87H ion exclusion column (Bio-Rad) as described in reference 66 (link).
Barthe M., Tchouanti J., Gomes P.H., Bideaux C., Lestrade D., Graham C., Steyer J.P., Meleard S., Harmand J., Gorret N., Cocaign-Bousquet M, & Enjalbert B. (2020). Availability of the Molecular Switch XylR Controls Phenotypic Heterogeneity and Lag Duration during Escherichia coli Adaptation from Glucose to Xylose. mBio, 11(6), e02938-20.
Acetate Adaptation Batch cultures BioreactorGlucose High performance liquid chromatographyXylose
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Université de Toulouse, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Centre National de la Recherche Scientifique, Institut National des Sciences Appliquées de Toulouse, École Polytechnique, Institut national de recherche en informatique et en automatique, Laboratoire de Biotechnologie de l'Environnement, Institut Universitaire de France
Nonlimiting aeration conditions obtained with an airflow set at 0.35 liter · min^-1 and adaptation of stirring to maintain pO2 of >20%
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