Colon sections were stained as described previously8 (link). The following antibodies were used: COX-2 (ab15191, 1:2000), STAT3 (CST 4904, 1:500), and CD3 (ab5690, 1:1000). Antibody binding was visualized with ImmPACT NovaRed (Vector Laboratories) and sections were counterstained with hematoxylin. Images were taken with a high resolution digital camera (Olympus DP 50) and analyzed by Cell^A imaging software (Olympus, Vienna, Austria). Only contrast and brightness of images were adjusted.
For cellular detection of GPR55 mRNA in colonic sections, RNAscope® 2.5 Chromogenic Assay with RNAscope® mouse GPR55 probe (#318231; ACD Advanced Cell Diagnostics, Hayward, CA, USA) was used according to the manufacturer’s instructions.
For cellular detection of GPR55 mRNA in colonic sections, RNAscope® 2.5 Chromogenic Assay with RNAscope® mouse GPR55 probe (#318231; ACD Advanced Cell Diagnostics, Hayward, CA, USA) was used according to the manufacturer’s instructions.
