Investigating ABCA1/ApoE in GW3965-induced neurorestoration after stroke

To investigate whether ABCA1/ApoE mediates GW3965-induced neurorestoration after stroke, ABCA1^fl/fl and ABCA1^−B/−B stroke mice were randomly divided into 4 groups by a non-team member using the method of drawing different colored balls. Mice were gavaged starting 24h after dMCAo with saline as vehicle control or GW3965 10mg/kg (Sigma) daily for 14 days based on previous dose-dependent study^{10 (link)}. After 14 days of treatment, animals were randomly separated into two sets: one set of animals (total 24 mice, n=6/group) were employed for WB and RT-PCR assay; the other set of animals (total 36 mice, n=9/group) were used for behavioral testing, blood-biochemistry, and lesion-volume and immunostaining measurements.
To investigate the effect of HDL on GW3965-induced neurorestoration, ABCA1^−B/−B stroke mice were randomly assigned to two groups (total 18 mice, n=9/group) and intraventricularly-infused with artificial cerebrospinal-fluid (CSF, Tocris Bioscience) 100μl as vehicle control, or human-plasma HDL3 (hHDL3, Cell Biolabs Inc.) 25μg in 100μl artificial-CSF by transplanting a micro-osmotic pump (D1002, Alzet) into the right lateral-ventricle initiated at 24h after dMCAo for 14 days. All mice were sacrificed 14 days after dMCAo.

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Cui X., Chopp M., Zhang Z., Li R., Zacharek A., Landschoot-Ward J., Venkat P, & Chen J. (2016). The ABCA1/ApoE/HDL pathway mediates GW3965-induced neurorestoration after stroke. Stroke, 48(2), 459-467.

Publication 2016

GW3965

Abca1 After treatment Animals Apoe Assay Blood Cell Cerebrospinal fluid Gw3965 Hdl3 Human Mice Osmotic Plasma Right ventricle Rt pcr Saline Stroke

Corresponding Organization :

Other organizations : Oakland University, Henry Ford Health System

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Variable analysis

independent variables

Treatment with saline as vehicle control or GW3965 10mg/kg daily for 14 days
Infusion of artificial cerebrospinal-fluid (CSF) or human-plasma HDL3 (hHDL3) 25μg in 100μl artificial-CSF for 14 days

dependent variables

Protein expression (Western blot) and gene expression (RT-PCR)
Behavioral testing
Blood biochemistry
Lesion volume and immunostaining measurements

control variables

ABCA1^fl/fl and ABCA1^-B/-B stroke mice
Randomization of mice into groups by a non-team member

positive controls

Not explicitly mentioned

negative controls

Saline as vehicle control for GW3965 treatment
Artificial CSF as vehicle control for HDL3 infusion

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