Herbal Decoction for Bioactive Compound Extraction

Eight raw herbs of BSHS were provided from the pharmacy department of the Wuqing Hospital of Traditional Chinese Medicine affiliated with Tianjin University of Traditional Chinese Medicine(Tianjin, China) (Supplementary Figure 1). Lysimachiae Herba, Lygodii Spora, Plantaginis Semen, Clerodendranthus Spicatus, Cibotii Rhizoma, Dipsaci Radix, Malva verticillata seed, and Licorice were mixed in proportions of 3:1.5:1.5:3:1.5:1.5:1.5:1(w/w) respectively and then soaked in 12 times the volume of distilled water (v/m) for 1 h, decocted twice, at 1.5 h per decoction. After concentrating the decoction to 1 g/mL, it was stored at -20°C until used.