Extraction and Preparation of Terminalia brownii Bark

A stem bark sample of T. brownii tree (Figure 1) was acquired from Kitui, Kenya (1.3099°S 37.7558°E; about 152 km from Nairobi) in May 2021, aided by a native herbalist. Botanical verification was conducted at the East African Herbarium situated at the National Museums of Kenya, and a voucher specimen was deposited there (accession number: JWM001). The plant sample was taken to the animal breeding and experimentation facility at Kenyatta University for preparation and bioassays. The sample was rinsed using running tap water, reduced into tiny shreds, and shade-dried till thoroughly dried and powdered [40 (link)].