Unilateral Renal Ischemia-Reperfusion Injury

Male mice ranging from 9 to 12 weeks of age (8 WT and 5 Cxcl4^−/−) were anesthetized by intraperitoneal injection (i.p.) with ketamine/xylazine (90 μg/g bodyweight (BW) ketamine, 9 μg/g BW xylazine). Analgesia was carried out by subcutaneous (s.c.) injection of metamizol (200 μg/g BW). For IRI procedure kidneys were exposed and mobilized by dorsolateral incision and perfusion was interrupted by clamping the renal artery using a non-traumatic microaneurysm clamp. Mice were kept at 37°C. After 28 minutes (min) ischemia, clamps were removed, and reperfusion was observed. The abdominal cavity was closed by peritoneal suture with prolene (6–0) and the skin clipped. For the sham procedure, skin and peritoneum were incised on the contralateral side, the kidney mobilized but not clamped, and the peritoneum and skin closed as described before. To minimize pain, metamizole (1.25 mg/mL) and 1% sucrose were added for three days to the drinking water. 28 days after IRI, mice were killed by cardiac puncture under ketamine/xylazine narcosis. The right ventricle was incised, the mouse was perfused with 30 mL PBS via the left ventricle and organs were taken for further analysis.³ (link)