The analytical column was directly connected to a 10 μm ZDV emitter (Bruker) inside of the Bruker captive source. The capillary voltage was set to 1700 V with the dry gas set at 3.0 L/min and 200° C. Data was acquired by diaPASEF on a Bruker timsTOF SCP with the ion accumulation and trapped ion mobility ramp set to 166 ms. DIA scans were optimized and acquired with 90 m/z windows spanning 300 to 1200 m/z and 0.6 to 1.43 1/K0. A 0.86 s cycle time included one full MS1 scan followed by 4 trapped ion mobility ramps to fragment all ions within the defined region.
Protocol detail
diaPASEF Analysis by timsTOF SCP
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Variable analysis
independent variables
- Capillary voltage
- Dry gas flow rate
- Dry gas temperature
- Ion accumulation and trapped ion mobility ramp duration
- DIA scan parameters (number of m/z windows, m/z range, 1/K0 range)
- Mass spectrometry data acquisition
- Analytical column connected to 10 μm ZDV emitter (Bruker) inside Bruker captive source
- No positive or negative controls were explicitly mentioned.
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