MDCK33016PF suspension cells (a proprietary cell line developed by Novartis31 (link)–33 (link) and now owned by Seqirus Limited) were maintained at densities between 1–1.5 × 106 cells/ml in 500 ml disposable spinner flasks (Corning, USA) in MDCK 33016 CDM (Lonza, Germany) at 37 °C, 4.5% CO2, shaking at 1 g. Cells were passaged at 3–4 day intervals.
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