Western Blot Analysis of Cellular Signaling

The antibodies specific against JUN (9165S), JUND (5000S), P-JUN Ser63 (2361S), P-JUN Ser73 (9164S), AKT (9272S), p-AKT Ser473 (9271S), RELA (8242S), PIK3CA (4255S), PARP (9542S), GAPDH (5174S), NFE2L2 (12721S), MTOR Pathway Antibody Sampler Kit (9964S), Autophagy Antibody Sampler Kit (4445S), RPS6KB1 Substrates Antibody Sampler Kit (2903S) and ULK1 Antibody Sampler Kit (8359T) were purchased from Cell Signaling Technology. Antibodies to GFP (sc-390394), ETS1 (sc-55581), SP1 (sc-14027) and MYC (sc-788) were bought from Santa Cruz Biotechnology. Antibodies that are specific against NFKB1 (ab32360) and SQSTM1 (ab155686) were bought from Abcam. Antibodies against ACTB (A1978) and TUBA/α-tubulin (T6199) were bought from Sigma Aldrich Corporation. Western blotting was performed as described in our previous publication.^73,74 (link)

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Huang H., Zhu J., Li Y., Zhang L., Gu J., Xie Q., Jin H., Che X., Li J., Huang C., Chen L.C., Lyu J., Gao J, & Huang C. (2016). Upregulation of SQSTM1/p62 contributes to nickel-induced malignant transformation of human bronchial epithelial cells. Autophagy, 12(10), 1687-1703.

Publication 2016

MTOR Pathway Antibody Sampler Kit Autophagy Antibody Sampler Kit MYC (sc-788) ab32360 ab155686

Antibodies Antibody Autophagy Gapdh Mtor Nfe2l2 Pik3ca Rela Ulk1 α tubulin

Corresponding Organization : New York University

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Protocol cited in 4 other protocols

Variable analysis

independent variables

JUN (9165S)
JUND (5000S)
P-JUN Ser63 (2361S)
P-JUN Ser73 (9164S)
AKT (9272S)
P-AKT Ser473 (9271S)
RELA (8242S)
PIK3CA (4255S)
PARP (9542S)
NFE2L2 (12721S)
GFP (sc-390394)
ETS1 (sc-55581)
SP1 (sc-14027)
MYC (sc-788)
NFKB1 (ab32360)
SQSTM1 (ab155686)

dependent variables

GAPDH (5174S)
ACTB (A1978)
TUBA/α-tubulin (T6199)

control variables

GAPDH (5174S)
ACTB (A1978)
TUBA/α-tubulin (T6199)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

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