Fluorescence microscopy of F-actin

The status of F-actin assembly in each group was determined as described previously (25 (link)). In brief, the samples from each group were incubated in 4% paraformaldehyde (PFA) for 10 min at room temperature and washed with PBS three times. Subsequently, the samples were immersed in Alexa Fluor 488 conjugated phalloidin reagents (Life Technologies, Waltham, MA, USA) at room temperature for 30 min. After being mounted on glass slides, the images were captured with a fluorescence microscope (Carl Zeiss, Weimar, Germany) and analyzed by ImageJ software (ImageJ 1.8, NIH, USA).

Free full text: Click here

Fan Y., Xiong Y., Wang X., Chen J., Fang D., Huang J, & Yuan G. (2023). Poly (lactic-co-glycolic acid)-encapsulated iodine-131 nanoparticles fabricated with rhTSH induce apoptosis and immobilization of thyroid cancer cells. Frontiers in Oncology, 13, 1030105.

Publication 2023

Alexa Fluor 488 conjugated phalloidin reagents fluorescence microscope

Alexa fluor 488 F actin Fluorescence microscope Paraformaldehyde Phalloidin

Corresponding Organization : Second Affiliated Hospital of Chongqing Medical University

Top 5 similar protocols

Variable analysis

independent variables

Incubation of samples in 4% paraformaldehyde (PFA) for 10 min at room temperature

dependent variables

Status of F-actin assembly

control variables

Washing of samples with PBS three times
Incubation of samples in Alexa Fluor 488 conjugated phalloidin reagents at room temperature for 30 min
Capturing of images with a fluorescence microscope
Analysis of images using ImageJ software

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!