Spade Mice Jak1 Mutation Protocol

Spade mice were kindly provided by H. Koseki (RIKEN Center for Integrative Medical Sciences, Laboratory for Developmental Genetics). Systemic Jak1 mutation in Spade mice was produced by N-ethyl N-nitrosourea (ENU) mutagenesis protocol. The detailed method was described previously (33 (link)). Mice were maintained in specific pathogen-free environment with a 12-h light/dark cycle with water and a standard diet (CLEA Rodent Diet CE-2; CLEA Japan, Tokyo, Japan) provided ad libitum. All experiments involving the use of animals were approved by and performed in accordance with the Guidelines for Animal Experimentation of the Animal Use Committee at Keio University.