Isolation and Characterization of hDPSCs

The protocol for the isolation, culture, and characterization of hDPSCs was evaluated by the bioethics committee of the ENES Leon Unit, UNAM, for authorization with the registration code CE_16 004_SN. We used permanent erupted third molars indicated for odontectomy in the ENES Leon Unit clinics of patients aged 16–25, free of pulpal and periapical pathology. The pulp tissue was isolated inside the horizontal laminar flow hood. The molar was cut at the level of the coronal–root junction with a low-speed turbine with a carbide disc with constant cooling until close to the pulp chamber. The pulp tissue was obtained and 1 × 1 mm explants were performed and incubated with minimum essential medium eagle medium (MEM, Sigma-Aldrich, Saint Louis, MO, USA) added with 20% fetal bovine serum (FBS, Gibco, USA), 1% glutamine (Gibco), and 1% antibiotic (Sigma-Aldrich) in Petri dishes 10 cm in diameter and incubated at 37 °C with 5% CO₂ and 95% humidity for 21 days