Thioglycollate-elicited macrophages were harvested by peritoneal lavage in sterile saline and pooled from 2–4 mice per strain per experiment as previously described(26 (link)). Cells were stimulated with E6020 at concentrations ranging from 0.1 ng/ml – 100 ng/ml for 2 hours after which macrophages were harvested in TriPure reagent (Roche, San Francisco, CA) and frozen at −80° C. RNA was extracted per the manufacturer’s protocol and cDNA was reverse transcribed from 1 μg RNA per sample using Qscript kits (QuantaBio, Beverly, MA)(26 (link)). qRT-PCR was performed on an ABI 7900HT instrument (Applied Biosystems, Waltham, MA) using Power SYBR Green PCR master mix (Applied Biosystems) with primers (Sigma-Aldridge, Inc., St. Louis, MO) for Hprt, Tnf, Il1b, Ifnb1, and Cxcl10(27 (link)). Levels of mRNA for specific genes are reported and statistical analyses performed on negative delta cycle threshold (CT) values, as normal distributions are preserved in this method. Data are displayed as arithmetic mean ± SEM. Conversion to relative gene expression normalized to that of WT unstimulated macrophages (“fold induction”)(28 (link)) are also shown on the right y-axis (Figure 1). The housekeeping gene encoding hypoxanthine-guanine phosphoribosyltransferase (Hprt) was used for normalization of RNA levels within each sample.