Macrophage Gene Expression Analysis
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Corresponding Organization : University of Maryland, Baltimore
Other organizations : Eisai (United States)
Variable analysis
- E6020 concentration (0.1 ng/ml - 100 ng/ml)
- MRNA levels of Hprt, Tnf, Il1b, Ifnb1, and Cxcl10
- Thioglycollate-elicited macrophages harvested by peritoneal lavage and pooled from 2-4 mice per strain per experiment
- RNA extraction and cDNA synthesis protocol
- QRT-PCR instrument (ABI 7900HT) and reagents (Power SYBR Green PCR master mix)
- Hprt as the housekeeping gene for normalization of RNA levels within each sample
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