Metabolite profiling analysis of the parental seedlings was performed as described previously (Dan et al., 2020 (link)). Briefly, untargeted metabolite profiles of 15-d-old seedlings were collected with a 1290 Infinity liquid chromatography system (Agilent Technologies, Santa Clara, CA, USA), Agilent quadrupole time-of-flight mass spectrometer (Agilent 6550 iFunnel QTOF; Agilent Technologies, Santa Clara, CA, USA), and Triple TOF 6600 mass spectrometer (AB SCIEX, Foster City, CA, USA). The metabolites were annotated using an in-house standard spectral library, and the lipids were annotated through matching with an in-house tandem mass spectrometry (MS/MS) spectral library. Data reliability was checked using a quality control sample, and the metabolite levels of a total of 3,746 detected analytes, among which 114 metabolites were annotated using the in-house spectral libraries, were normalized (sum, log, and none) for the statistical analyses.
Protocol detail
Untargeted Metabolite Profiling of Seedlings
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Wuhan University
Other organizations : Washington State University
Top 5 similar protocols
Variable analysis
independent variables
- None explicitly mentioned
- Metabolite levels of a total of 3,746 detected analytes, among which 114 metabolites were annotated using the in-house spectral libraries
- Quality control sample used to check data reliability
- A quality control sample was used to check the reliability of the data
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!