Quantitative Immunohistochemistry of Ki67

Tumors were processed for immunohistochemistry as previously described (24 (link)). Ki67 (CST #9027, 1:400) antibody was used and bound antibodies were detected using the VECTASTAIN Universal Kit/HRP (Vector Laboratories) and 3,3’-diaminobenzidine substrates. Images are shown at a magnification of 20X and were quantified in three separate areas by counting the number of positive cells and creating an average and standard error that is presented in graphic form.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

McDaniel N.K., Iida M., Nickel K.P., Longhurst C.A., Fischbach S.R., Rodems T.S., Kranjac C.A., Bo A.Y., Luo Q., Gallagher M.M., Welke N.B., Mitchell K.R., Schulz A.E., Eckers J.C., Hu R., Salgia R., Hong S., Bruce J.Y., Kimple R.J, & Wheeler D.L. (2020). AXL mediates cetuximab and radiation resistance through tyrosine 821 and the c-ABL kinase pathway in head and neck cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 26(16), 4349-4359.

Publication 2020

VECTASTAIN Universal Kit/HRP

Antibodies Antibody Immunohistochemistry Tumors Vector

Corresponding Organization : University of Wisconsin Carbone Cancer Center

Other organizations : City of Hope, Yonsei University

Top 5 similar protocols

Variable analysis

independent variables

Tumor processing for immunohistochemistry

dependent variables

Ki67 positive cells
Average and standard error of Ki67 positive cells

control variables

Antibody used (Ki67, CST #9027, 1:400)
Detection method (VECTASTAIN Universal Kit/HRP, Vector Laboratories)
Substrate used (3,3'-diaminobenzidine)
Magnification (20X)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!