In parallel, another blood sample was processed using the Parsortix system at 99-mbar through a 6.5-µm cassette (
CTC Isolation, Immunostaining, and PD-L1 Detection
In parallel, another blood sample was processed using the Parsortix system at 99-mbar through a 6.5-µm cassette (
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Corresponding Organization : Edith Cowan University
Other organizations : Linear Clinical Research, Sir Charles Gairdner Hospital, University of Western Australia, Fiona Stanley Hospital, Harry Perkins Institute of Medical Research
Protocol cited in 2 other protocols
Variable analysis
- CTC enrichment method (anti-EpCAM coated magnetic beads vs. Parsortix system)
- CTC capture and immunostaining
- PD-L1 expression
- Centrifugation speed and duration for plasma isolation
- Blood sample volume (8-9 mL) processed through each method
- Positive controls: MCF7 cell line, MCF7 cells induced with IFN-γ, MDA-MB-231 cell line spiked into white blood cells from healthy donors
- Negative control: Not explicitly mentioned
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