Filament solutions of the three PAs were diluted to 500 μM before nebulization, and then 3 mL of each PA was added to the nebulizer reservoir. During nebulization, solutions were collected at 2 min time intervals over the course of 10 min from the nebulized mist and reservoir to analyze. Samples were diluted to 100 μM and placed in a UV-transparent disposable cuvettes ZEN0118 composed of polystyrene latex (Sarstedt, Germany) to then run on a Malvern ZEN3690 Zetasizer (Malvern Panalytical, Westborough, MA) at 25 °C. Since the software uses models for fitting of spherical particles and the structures in our samples are anisotropic, we used the molar scattering rate (scattering rate normalized to sample concentration, Mcounts/s/M) as a measure of assembly size.71 (link),73 (link) Three runs were collected for each sample (10 scans/measurement) and averaged. The molar scattering rates were normalized to the 0 min time point.
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Nebulization and Size Analysis of PAs
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Corresponding Organization : Johns Hopkins Medicine
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Variable analysis
independent variables
- Time intervals over the course of 10 min from the nebulized mist and reservoir
- Molar scattering rates of the samples
- Filament solutions of the three PAs were diluted to 500 μM before nebulization
- 3 mL of each PA was added to the nebulizer reservoir
- Samples were diluted to 100 μM before measurement
- Measurements were conducted at 25 °C
- Molar scattering rates were normalized to the 0 min time point
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