As previously described (19 (link)), muscle RNA was extracted from 10-20 mg of skeletal muscle tissue. These samples were homogenized in TRI Reagent® (Sigma, St Louis, Missouri) and further isolated using an RNA isolation kit, according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). RNA concentration and purity were assessed by measuring absorbance at 230, 260, 280 nm, using the NanoDrop ND-1000 Spectrophotometer (Thermo Scientific, Wilmington, Delaware). Random assessments of RNA integrity were performed using an Agilent bioanalyzer (Agilent, Santa Clara, CA).
Protocol detail
Skeletal Muscle RNA Extraction Protocol
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Corresponding Organization :
Other organizations : Pennington Biomedical Research Center, Louisiana State University, Case Western Reserve University, Western University of Health Sciences
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Variable analysis
independent variables
- Amount of skeletal muscle tissue (10-20 mg)
- RNA concentration
- RNA purity (assessed by absorbance at 230, 260, 280 nm)
- RNA integrity (assessed by Agilent bioanalyzer)
- Homogenization method (TRI Reagent®)
- RNA isolation kit (Bio-Rad)
- Spectrophotometer (NanoDrop ND-1000)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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