The assay was conducted according to the protocols described in our previous studies6 (link),25 (link). Primary antibodies include: FZD7 (1:1000, ab64636, Abcam, MA, USA), β-catenin (1:1000, ab32572, Abcam), Cyclin D1 (1:1000, ab134175, Abcam), c-Myc (1:1000, ab32072, Abcam), and β-actin (1:1000, ab8226, Abcam). And Lamin B1 (1:5000, ab194109, Abcam) served as a nuclear internal control.
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