Fluorescent Albumin-Based Brain Microvasculature Imaging

A microangiographic technique based on the injection of fluorescent probes enables visualization of the entire brain microvasculature [25 (link),26 (link)]. To this end, fluorescent albumin was used, since albumin does not extravasate abluminally if the BBB is intact [25 (link),26 (link)]. In the present study, the fluorescent albumin solution was prepared by reconstituting 500 mg of bovine desiccate albumin-fluorescein isothiocyanate (FITC-albumin, MW 69 kDa; Sigma, St. Louis, MO) in 50 mL of phosphate buffered saline (0.1M PBS) lacking magnesium and calcium ions. FITC-albumin was directly infused in the left ventricle [25 (link),26 (link)]. 10 mL of solution were injected at a rate of 1 mL/min under general anesthesia. The presence of vascular leakage in treated and control animals was evaluated by visual inspection and by fluorescent microscopy.

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Ghosh C., Myers R., O’Connor C., Williams S., Liu X., Hossain M., Nemeth M, & Najm I.M. (2022). Cortical dysplasia in rats provokes neurovascular alterations, GLUT1 dysfunction and metabolic disturbances that are sustained post-seizure induction. Molecular neurobiology, 59(4), 2389-2406.

Publication 2022

FITC-albumin

Albumin Animals Bovine Brain Calcium Desiccate Fitc albumin Fluorescein Fluorescent probes General anesthesia Ions Isothiocyanate Left ventricle Magnesium Microscopy Microvasculature Phosphate Saline Vascular

Corresponding Organization : Cleveland Clinic Lerner College of Medicine

Other organizations : Cleveland Clinic

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Variable analysis

independent variables

Injection of fluorescent albumin solution

dependent variables

Presence of vascular leakage in treated and control animals (evaluated by visual inspection and fluorescent microscopy)

control variables

Bovine desiccate albumin-fluorescein isothiocyanate (FITC-albumin, MW 69 kDa; Sigma, St. Louis, MO) reconstituted in 50 mL of phosphate buffered saline (0.1M PBS) lacking magnesium and calcium ions
FITC-albumin directly infused in the left ventricle at a rate of 1 mL/min under general anesthesia

controls

Treated animals (received FITC-albumin injection)
Control animals (did not receive FITC-albumin injection)

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