Briefly, the tissues that were previously formalin-fixed and paraffin-embedded were sliced into 4 µm sections, and were then incubated with the primary antibody recognizing human DAB2IP (#ab87811, abcam) at 1:200 dilution at 4°C overnight. The protein was visualized using a tissue staining kit (Zhongshan Biotechnology, Beijing, China) according to the manufacturer’s instructions. The final staining score was determined by color intensity and positive cell rate, ranging 0–12 which was described in our previous study.23 (link),24 (link) Patients were classified into two groups: scores 0–4 were considered as none or low, while 5–12 were considered as high expression.
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Immunohistochemical Analysis of DAB2IP
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Other organizations : First Affiliated Hospital of Wannan Medical College
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Variable analysis
independent variables
- Primary antibody recognizing human DAB2IP (#ab87811, abcam)
- Final staining score determined by color intensity and positive cell rate, ranging 0–12
- Tissue samples were formalin-fixed and paraffin-embedded
- Tissue sections were 4 µm thick
- Primary antibody was used at 1:200 dilution
- Primary antibody incubation was at 4°C overnight
- Protein visualization was done using a tissue staining kit (Zhongshan Biotechnology, Beijing, China) according to the manufacturer's instructions
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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