Western Blot Analysis of Cell Signaling

A Western blot analysis of whole-cell lysates was conducted as previously described [17 (link)]. The blots were probed with the following primary antibodies: phosphor-CDK2, total CDK2, CDK3, phosphor-CDC2, total CDC2, phospho-ERK1/2, phosphor-JNK1/2 (all from Cell Signaling), CDK4 (Santa Cruz Biotechnology), total p38, total ERK1/2, total JNK1/2, phospho-p38, total p38 MAPK (all from Promega), anti α-tubulin (Sigma) and anti-LC3 (MBL). Immunoreactive proteins were detected as previously described [17 (link)].

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Sugimori N., Espinoza J.L., Trung L.Q., Takami A., Kondo Y., An D.T., Sasaki M., Wakayama T, & Nakao S. (2015). Paraptosis Cell Death Induction by the Thiamine Analog Benfotiamine in Leukemia Cells. PLoS ONE, 10(4), e0120709.

Publication 2015

total CDK2 phosphor-CDC2 total CDC2 phospho-ERK1/2 phosphor-JNK1/2 total ERK1/2 phospho-p38 anti α-tubulin

Antibodies Cdc2 Cdk2 Cdk3 Cell Erk1 P38 mapk Phosphor Promega Proteins Western blot analysis α tubulin

Corresponding Organization : Kanazawa University

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Variable analysis

independent variables

None explicitly mentioned.

dependent variables

Phosphorylation levels of CDK2, CDC2, ERK1/2, JNK1/2, and p38 MAPK
Levels of total CDK2, CDK3, CDC2, ERK1/2, JNK1/2, and p38 MAPK
Levels of α-tubulin and LC3

control variables

Western blot analysis of whole-cell lysates was conducted as previously described [17 (link)]
Immunoreactive proteins were detected as previously described [17 (link)]

controls

Positive controls: None specified
Negative controls: None specified

Annotations

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