Hypoxic Response in Pulmonary Cells

Human pulmonary artery endothelial cells (HPAEC; Promocell, C-12241, lot 7121003.5 and 4091902) and smooth muscle cells (HPASMCs; Promocell, C-12521, lot 9033101.6) were cultured, as described in Ref. 4 (link). The cells were used between passages 3–6. The cells were kept under normoxia or were exposed to hypoxia (2% O₂) for 2–72 h. In experiments involving a short-term (2 h) exposure to hypoxia, tipifarnib (Selleckchem) was added to the cells 1 h before the hypoxic exposure at 0.1 µmol/L. In experiments involving a more prolonged exposure to hypoxia (24–72 h), tipifarnib was added to the cells at the start of hypoxic exposure.⁴ (link)