Cold Exposure Body Composition Study

Body composition and body temperature data were obtained as we previously described [16 (link)]. Whole-body composition was monitored using Echo MRI-100 whole-body composition analyzer (Echo Medical Systems, Houston, TX). For the cold exposure study, mice were individually caged in 4°C cold room for 4‒6 h, with free access to food and water. Core body temperature was measured with a rectal probe attached to a TH-8 Thermalert monitoring thermometer (Physitemp Instruments Inc. Clifton, NJ). Body temperature was assessed hourly for the duration of the experiment. For humane consideration required by IACUC policy, the experiment was aborted when a temperature lower than 25°C detected in the mice.

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Lee J.H., Lin L., Ye X., Wolfrum C., Chen Y., Guo S, & Sun Y. (2021). GHS-R in brown fat potentiates differential thermogenic responses under metabolic and thermal stresses. PLoS ONE, 16(4), e0249420.

Publication 2021

Echo MRI-100 whole-body composition analyzer TH-8 Thermalert monitoring thermometer

Body composition Cold Echo Food Iacuc Mice Rectal Thermometer

Corresponding Organization : University of Mississippi Medical Center

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Variable analysis

independent variables

Cold exposure (4°C)

dependent variables

Body composition
Core body temperature

control variables

Free access to food and water during cold exposure
Rectal probe for measuring core body temperature
Experiment aborted when core body temperature dropped below 25°C

controls

Positive control: None mentioned
Negative control: None mentioned

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